CRISPR screening by AAV episome-sequencing (CrAAVe-seq): a scalable cell-type-specific in vivo platform uncovers neuronal essential genes

Nature Neuroscience, Published online: 22 August 2025; doi:10.1038/s41593-025-02043-9

The authors developed an adeno-associated virus-based high-throughput in vivo CRISPR screening platform for endogenous mouse brain cell types. Using this platform, they define genes and pathways essential for neuronal survival.